The living cell is a magnificently orchestrated molecular system that uses precisely controlled chemistry to interact with its environment and control its inner workings. The details of this chemistry are exquisitely challenging to study in a test tube, and understanding these processes in living cells requires methods for real time visualization. We design highly chemoselective transformations that are operative inside of living organisms and use streamlined synthetic protocols for the preparation of fluorescence microscopy imaging probes. Our particular interest lies in developing methods to detect and image reactive small molecule species derived from sulfur, oxygen, nitrogen, and carbon. Organic synthesis and reaction methodology are core technologies utilized in our lab, which we use both to develop new types of imaging agents as well as optimize and characterize existing agents. Untangling the details of cellular chemistry will require a combination of imaging agents and techniques and students in my lab learn a diverse set of skills ranging from organic synthesis to cellular biology.
Collaborators: Prof. Peng Tao, Department of Chemistry, SMU
Prof. Thomas Ritz, Department of Clinical Psychology, SMU
Reeves, A. G.; Subbarao, M.; Lippert, A. R. "Imaging acetaldehyde formation during ethanol metabolism in living cells using a hydrazine naphthalimide fluorescent probe." Anal. Methods 2017, 9, 3418-3421. doi:10.1039/C7AY01238A
Bruemmer, K. J.; Merrikhihaghi, S.; Lollar, C. T.; Morris, S. N. S.; Bauer, J. H.; Lippert, A. R. "19F Magnetic Resonance Probes for Detecting Peroxynitrite in Living Cells Using an Oxidative Decarbonylation Reaction." Chem. Commun. 2014 , 50, 12311. doi: 10.1039/C4CC04292A
Lollar, C. T.; Krenek, K. M.; Bruemmer, K.J.; Lippert, A. R. "Ylide Mediated Carbonyl Homologations for the Preparation of Isatin Derivatives."
Org. Biomol. Chem.
, 12, 406. doi: 10.1039/C3OB42024H.
Lin, V. S.; Lippert, A. R.; Chang, C. J. "Cell-trappable fluorescent probes for endogenous hydrogen sulfide signaling and imaging H2O2-dependent H2S production."
Proc. Natl. Acad. Sci. USA
, 110, 7131. doi: 10.1073/pnas.1302193110 (Highlighted in the April 22 issue of Chemical & Engineering News)