The Lippert Research Group

Triggered Chemiluminescence for Whole Animal Imaging

Chemiluminescence, the generation of light from a chemical reaction, does not require an excitation source and eliminates autofluorescence and light scattering that is endemic to fluorescence imaging. Indeed, bioluminescence, one type of chemiluminescence that uses an enzymatic reaction to produce light, has become a powerful pre-clinical imaging technique, but requires genetically modified animals that express bioluminescent enzymes. In order to circumvent this key obstacle and open up the possibility of high throughput preclinical imaging of wild-type animals, our lab is developing innovative triggered energy transfer chemiluminescence agents for imaging a range of molecular markers. We are working towards next generation imaging agents for quantitative imaging of the tumor microenvironment

Collaborators:
Prof. Ralph Mason, Department of Radiology, UTSW
Prof. Li Liu, Department of Radiology, UTSW
Prof. David Son, Department of Chemistry, SMU

Cao, J.; Lopez, R.; Thacker J. M.; Moon, J. Y.; Jiang, C.; Morris, S. N. S.; Bauer, J. H.; Tao, P.; Mason, R. P.; Lippert, A. R.* "Chemiluminescent Probes for Imaging H2S in Living Animals." Chem. Sci. 2015 , 6, 1979. doi: 10.1039/C4SC03516J.

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Cao, J.; Campbell, J.; Liu, L.; Mason, R. P.; Lippert, A. R.* "In Vivo Chemiluminescent Imaging Agents for Nitroreductase and Tissue Oxygenation." Anal. Chem. 2016, 88, 4995. doi:10.1021/acs.analchem.6b01096

TOC Graphic CHS3.jpg